Genotyping ocular strains of Chlamydia trachomatis by single-tube nested PCR.

Genotyping of Chlamydia trachomatis from endocervical specimens in Shiraz, Iran

The objective of this study was to determine the prevalence and distribution of Chlamydia trachomatisgenotypes in Shiraz, Iran. Two hundred twelve cervical swab samples were collected from women attendingShahid Motahari Polyclinic in Shiraz, Iran. The endocervical specimens were screened for C. trachomatis byplasmid PCR. Genotyping was performed in C. trachomatis-positive samples by nested PCR ...

Genotyping of Endocervical Chlamydia trachomatis Strains and Detection of Serological Markers of Acute and Chronic Inflammation in Their Host

chlamydia trachomatis detection by nested-pcr method on females referred to medical centers of tehran, iran

conclusions: nested pcr as a sensitive chlamydia trachomatis detection test and endocervical specimens has been offered to detect this bacterium in spontaneous abortion. besides, c. trachomatis screening among pregnant women can be suggested to prevent abortion. results: the number of intercourses per week and history of miscarriage can be known as the risk factors of abortion. frequency of c. ...

High-resolution genotyping of Chlamydia trachomatis strains by multilocus sequence analysis.

Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multilocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determi...

Genotyping of Portuguese Chlamydia trachomatis urogenital isolates.

OBJECTIVE To determine the prevalence of the different Chlamydia trachomatis genotypes in Portuguese patients. METHODS Urogenital isolates (n = 240) derived from attenders of various clinics in the Lisbon area were differentiated into genovars by genotyping with restriction fragment length polymorphism (RFLP) analysis of the PCR amplified omp1 gene. RESULTS Genotype E was the most common fo...