Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

Analysis of potyvirus terminal protein VPg-transgenic Arabidopsis thaliana plants.

Virus-coded VPg protein of Potato virus Y (PVY) does not have homologs apart from other VPgs. Since VPg is indispensable for the potyvirus life cycle, it appeared a good candidate for eliciting pathogen-derived resistance to PVY. Following agroinfection used to obtain PVY VPg-transgenic Arabidopsis thaliana plants, only few transgenic seeds were recovered giving rise to six transgenic plants th...

Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.

The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a substantially modified transformation method was developed. The labor-intensive vacuum infiltration process was eliminated in favor of simple dipping of developing floral tissues into a solution co...

studying the efficiency of floral dip method for genetic transformation of apiaceae plants

plant tissue culture techniques are used as basic requirement of common plant transformation systems. in most cases of plant transformation, a reproducible regeneration protocol is the limiting step due to long time lasting, specialized facilities and well experienced persons. furthermore, tissue culture procedures induce somaclonal variation among regenerated transgenic plants. therefore, rece...

Genomic Analysis of Arabidopsis Thaliana

construction of pbi121: expb2 and its transformation into arabidopsis thaliana

plant growth is a result of cell division and cell enlargement. expansins are proteins that mediate long-term extension of isolated cell walls and they play a variety of roles in plant cells. in this study the atexpb2 gene was over-expressed in arabidopsis thaliana. the gene was isolated from genomics dna of arabidopsis and cloned into mediated vectors; pgemt and pbi121 vectors. transformation ...