Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology
نویسندگان
چکیده
منابع مشابه
Propranolol induced chromosomal aberrations in Chinese hamster ovary cell line
Propranolol (PL), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. The present study was concerned with assessing the cytogenetic effects of this drug on Chinese hamster ovary (CHO) cell line. MTT assay was then carried out to determine the cytotoxicity index (IC50) of the drug. The IC50 value of PL was 0.43±0.02 mM. To investigate the clastogenic effect...
متن کاملMutation Detection in an Antibody-Producing Chinese Hamster Ovary Cell Line by Targeted RNA Sequencing.
Chinese hamster ovary (CHO) cells have been used widely in the pharmaceutical industry for production of biological therapeutics including monoclonal antibodies (mAb). The integrity of the gene of interest and the accuracy of the relay of genetic information impact product quality and patient safety. Here we employed next-generation sequencing, particularly RNA-seq, and developed a method to sy...
متن کاملpropranolol induced chromosomal aberrations in chinese hamster ovary cell line
propranolol (pl), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. the present study was concerned with assessing the cytogenetic effects of this drug on chinese hamster ovary (cho) cell line. mtt assay was then carried out to determine the cytotoxicity index (ic50) of the drug. the ic50 value of pl was 0.43±0.02 mm. to investigate the clastogenic effect...
متن کاملA simple method for directional transcriptome sequencing using Illumina technology
High-throughput sequencing of cDNA has been used to study eukaryotic transcription on a genome-wide scale to single base pair resolution. In order to compensate for the high ribonuclease activity in bacterial cells, we have devised an equivalent technique optimized for studying complete prokaryotic transcriptomes that minimizes the manipulation of the RNA sample. This new approach uses Illumina...
متن کاملcloning and expression of luteinizing hormone subunits in chinese hamster ovary cell line
background: luteinizing hormone (lh) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. the application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively. m aterials and methods: in the present study the alpha and beta subunits of human lh gene were cloned into the pegfp-n1 expression vector and produc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Genomics
سال: 2011
ISSN: 1471-2164
DOI: 10.1186/1471-2164-12-67