Genomic DNA extraction and amplification of Leishmania donovani using polymerase chain reaction (PCR) from archived, Giemsa- stained slides
نویسندگان
چکیده
منابع مشابه
DNA extraction and amplification of leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous Leishmaniasis by PCR.
DNA was isolated from 92 Giemsa-stained smears of lesions from suspected cases of cutaneous leishmaniasis and used for PCR-based diagnosis of Leishmania infection. Each smear had been examined under a light microscope at x 1,000 and scored for amastigote numbers. Although the smears had been stored for up to 4 years, all the microscopy-positive slides were also positive by PCR and four of the 1...
متن کاملIdentification of Leishmania Species Using PCR Assay on Giemsa-Stained Slides Prepared From Cutaneous Leishmaniasis Patients
BACKGROUND Leishmaniasis is a group of diseases that are created by intracellular parasites of Leishmania. Cutaneous leishmaniasis is considered as one of the health problems in some provinces of Iran. METHODS In this study, a total of 178 Giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. The slides were prepared from the patients with cutaneous leishmaniasi...
متن کاملidentification of leishmania species using pcr assay on giemsa-stained slides prepared from cutaneous leishmaniasis patients.
background: leishmaniasis is a group of diseases that are created by intracellular parasites of leishmania. cutaneous leishmaniasis is considered as one of the health problems in some provinces of iran. methods: in this study, a total of 178 giemsa-stained slides from confirmed cases of cutaneous leishmaniasis were examined. the slides were prepared from the patients with cutaneous leishmaniasi...
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Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
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ژورنال
عنوان ژورنال: Sri Lankan Journal of Infectious Diseases
سال: 2021
ISSN: 2448-9654,2012-8169
DOI: 10.4038/sljid.v11i1.8323