Genome of Bacteriophage P1

Genome of bacteriophage P1.

P1 is a bacteriophage of Escherichia coli and other enteric bacteria. It lysogenizes its hosts as a circular, low-copy-number plasmid. We have determined the complete nucleotide sequences of two strains of a P1 thermoinducible mutant, P1 c1-100. The P1 genome (93,601 bp) contains at least 117 genes, of which almost two-thirds had not been sequenced previously and 49 have no homologs in other or...

Genome of Bacteriophage P1 Supplemental Material

Curing of P1 prophage from Escherichia coli K-12 recA(P1) lysogens superinfected with P1 bacteriophage.

Curing of the P1 plasmid prophage in recA(P1) lysogens by superinfection with another P1 phage was specific and independent of immunity and incompatibility expression.

Bacteriophage P1 in retrospect and in prospect.

In consenting to publish an extravagantly long report of the most tedious kind—a genome analysis (10)—this journal has shown what appears to be extraordinary indulgence. The genome is that of P1, a bacteriophage that, unlike certain of its cousins, neither raises hopes nor instills fear. P1 phage particles are not the “great hope of universal therapy and prophylaxis,” as Felix d’Hérelle believe...

Three functions of bacteriophage P1 involved in cell lysis.

Amber and deletion mutants were used to assign functions in cell lysis to three late genes of bacteriophage P1. Two of these genes, lydA and lydB of the dar operon, are 330 and 444 bp in length, respectively, with the stop codon of lydA overlapping the start codon of lydB. The third, gene 17, is 558 bp in length and is located in an otherwise uncharacterized operon. A search with the predicted ...