Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato

Potato is an important food crop worldwide and grown in a large number of countries. As such, the under disease pressures need for selecting resistance genes during breeding programs essential. Of particular importance within Australia other parts world potyvirus, virus Y (PVY). In this paper, three commonly used PVY markers, M45, RYSC3 M6, were evaluated using existing genomic resources phenotypic data from Australian potato program to identify region where gene, Ryadg may reside. A Chromosome XI was investigated, cluster identified that gene suspected reside within. Protein characterization also performed on putative resistant gene. specific variant had complete association with single nucleotide polymorphism (SNP) assay designed avoid dissociation marker future programs. This SNP (SNP37279) validated as Kompetitive Allele-specific PCR (KASP) genotyping found perform more accurately than all previously markers detecting Ryadg.