Genetics of Radiation Sensitivity in the Slime Mould Dictyostelium discoideum
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چکیده
منابع مشابه
Genetic analysis of radiation-sensitive mutations in the slime mould Dictyostelium discoideum.
The linkage of two mutations leading to increased sensitivity to ultraviolet light and 60Co gamma rays was determined in the slime mould Dictyostelium discoideum using a genetic analysis based on the parasexual cycle. Diploids were selected from a mixture of radiation-sensitive, temperature-resistant and radiation-resistant, temperature-sensitive haploids on the basis of simultaneous radiation ...
متن کاملExperimentally induced aberrations in the pattern of differentiation in the cellular slime mould Dictyostelium discoideum.
This paper describes a set of perturbatory experiments designed to elucidate aspects of the mechanism by which the normal pattern of differentiation is specified. Experiments are described which investigate the alterations in development seen in aqueous environments, with changes in humidity and with the introduction of permeable and impermeable barriers. The following results are reported: (i)...
متن کاملGrowth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
1. A simple axenic medium suitable for the growth of the myxamoebae of a strain of the cellular slime mould Dictyostelium discoideum is described. 2. Procedures suitable for the growth of this strain in liquid and on solid media are described. 3. Conditions suitable for initiating the cell differentiation of myxamoebae grown axenically are described.
متن کاملTwo dephosphorylation pathways of inositol 1,4,5-trisphosphate in homogenates of the cellular slime mould Dictyostelium discoideum.
Dictyostelium discoideum homogenates contain phosphatase activity which rapidly dephosphorylates Ins(1,4,5)P3 (D-myo-inositol 1,4,5-trisphosphate) to Ins (myo-inositol). When assayed in Mg2+, Ins(1,4,5)P3 is dephosphorylated by the soluble Dictyostelium cell fraction to 20% Ins(1,4)P2 (D-myo-inositol 1,4-bisphosphate) and 80% Ins(4,5)P2 (D-myo-inositol 4,5-bisphosphate). In the particulate frac...
متن کاملControl of cellular differentiation by temperature in the cellular slime mould Dictyostelium discoideum.
The effects of low temperature on morphogenesis and cellular differentiation of Dictyostelium discoideum were examined. During incubation at 5 degrees C, the vegetative and preaggregation cells never developed, but cell masses at the aggregation or slug stage developed to form hemispherical, or dumbbell-shaped multicellular structures. By staining with FITC-antispore IgG, the structures formed ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1978
ISSN: 0022-1287
DOI: 10.1099/00221287-109-1-11