Genetic variation of CAST gene in Local Karnobat and Karnobat merino sheep breeds

Karnobat sheep plays an important role in the development of breeding Southeastern region Bulgaria. They are valuable source genetic material. The aim present experiment was to determine allele variation CAST gene Local and Merino breeds. A total 60 blood samples were collected – 30 per breed. DNA extracted genotypes all animals identified by means PCR-RFLP technique. restriction reactions accomplished specific enzyme MspI . As expected both breeds characterized with low level diversity due fact that mostly maintaining selection has been implemented. In breed only one heterozygous individual from 30. merino M frequency 0,97 N 0,03. Genotypes MM MN revealed frequencies 0,93 0,07, respectively. According statistical analysis HWE equilibrium.