GENETIC VARIABILITY OF FAGOPYRUM SP. GENOTYPES DETERMINED BY GENE-TARGETED MARKERS

Fagopyrum represents an economically and nutritionally important crop which belongs to the group of pseudocereals. Buckwheat is referred be a functional with wide range use around world. Grains buckwheat are significant. They characterised by high content starch, proteins, flavonoids fibre. SCoT technique used for detection plant genes polymorphism, evaluation genetic diversity mapping regions genome. The aim work was perform molecular analyses 21 genotypes esculentum Moench 14 tataricum Gaertn. using ten gene-targeted markers. seedlings were DNA isolation subsequently amplification polymerase chain reaction. number amplified fragments ranged from 12 (SCoT 60, 13) 27 12). total 176 162 polymorphic average 16.2. percentage polymorphism 58.33 % 60) 100 12, 13, 29, 30) 90.29 %. Polymorphic information (PIC) values characterize PIC 0.578 0.932 36) 0.859. determined hierarchical cluster analysis UPGMA algorithm in created dendrogram. Genotypes divided into two main clusters. Two common (Siva, Špačinska I) Slovenia Slovak Republic, respectively, as well another tartary (Tohno Zairai, Winsor Royal) unknown regions, genetically closest. PCoA plot confirmed separation resulting constructed dendrogram According our results, we can consider appropriate differentiation leading genotype identification utilization breeding process improve material.