Genetic transformation of the plant pathogens Phytophthora capsici andPhytophthora parasitica

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Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.

Phytophthora capsici and P.parasitica were transformed to hygromycin B resistance using plasmids pCM54 and pHL1, which contain the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polye...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1991

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/19.15.4273