Genetic mapping of tolerance to iron deficiency chlorosis in peanut (Arachis hypogaea L.)

Iron deficiency chlorosis (IDC) under calcareous and alkaline soils is a significant abiotic stress affecting the growth yield of peanut. In this study, genomic regions governing IDC tolerance were mapped using recombinant inbred line (RIL) population derived from TMV 2 (susceptible to IDC) 2-NLM (tolerant IDC), which was phenotyped during rainy seasons 2019 2020 in iron-deficient plots. The best linear unbiased prediction (BLUP) values for traits like visual chlorotic rating (VCR), SPAD chlorophyll meter reading (SCMR) used QTL analysis along with genetic map carrying 700 GBS-derived SNP, AhTE SSR markers. total, 11 12 main-effect QTLs identified VCR SCMR, respectively. Among them three major phenotypic variance explained (PVE) 10.3–34.4% VCR, two SCMR PVE 11.5–11.7%. A region (159.3–178.3 cM) on chromosome Ah13 (one each SCMR) consistent previous report. SNP marker, Ah14_138037990 single marker located intronic gene Arahy.QA0C1, important protein-binding. Overall, study new also validated tolerance. These resources could be useful genomics-assisted breeding peanut