Genetic locus copy number variation level and minimally invasive assessment of radiation therapy efficiency in rectal cancer patients

Aim. The screening of molecular genetic markers for a minimally invasive assessment the radiation therapy effectiveness rectum malignant tumors. Materials and methods. study was carried out in 4 stages: 1) bioinformatic analysis TCGA (The Cancer Ge- nome Atlas) databases using GISTIC algorithm; 2) validation bioinformatics data model experiment on cell culture; 3) genes copy number features validated patients with different responses to therapy; 4) determination gene cell-free DNA patientswith therapy. 100 adenocarcinoma (G1–2), as well 30 apparently healthy individuals, took part work. Radiotherapy according standard protocol (single focal dose 2.4 Gy, total 54 Gy) Novalis TX linear accelerator. relative loci determined by real-time quantitative polymerase chain reaction. Results. Bioinformatic revealed 32 candidate loci. Validation these irradiated HT-29 cells showed that BRCA2 , H2AX CASP9 RBBP8 increased, while BCL2 reduced intact cells. In 74 partial response therapy, an increase found, which positively correlated blood plasma DNA. Conclusion. application integrated approach based database analysis, modeling culture identified tissue samples rectal association preoperative effectiveness.