Genetic diversity study of Chrysoperla carnea (Neuroptera: Chrysopidae) populations via molecular markers

The objective of this study was to determine the genetic diversity among Chrysoperla carnea samples collected from different locations Iran (including, East-Azerbaijan, West-Azerbaijan, Isfahan, Kerman, Kermanshah, Lorestan, Mazandaran, Gilan, Hormozgan and Hamedan provinces) using Inter simple sequence repeat (ISSR) mitochondrial (Cytochrome Oxidase I - COI) molecular markers in 2016-2018. results showed that a total 64 bands were produced by ten primers ISSR which them 43 polymorphic. highest lowest polymorphic percentages belonged primer UBC-809 (88.88%) UBC-886 (33.33%), respectively. cluster analysis based on marker data divided into three separate clusters. This grouping also confirmed variance. According variance within groups about 84% 16%, In present five haplotypes obtained. first haplotype (H1) common all populations can be considered as ancestral haplotype, other have been evolved it. novelty is we report time family Chrysopidae CO1 covering more than provinces thirty cities with full picture its diversity. Genetic distance matrix Jaccard index indicated low populations. high efficiency Chrysopidae.