Genetic Characterization of AmpC and Extended-Spectrum Beta-Lactamase Phenotypes in Escherichia coli and Salmonella From Alberta Broiler Chickens

Horizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 Escherichia coli and 62 Salmonella enterica subsp. isolates were isolated from broiler chicken farms Alberta. Fourteen serovars identified among isolates. Thirty one percent E. (37/120) multiclass drug resistant (resistant to ? 3 classes), while only about 16% (10/62) resistant. Among those, eight had AmpC-type phenotype, isolate extended-spectrum beta-lactamase (ESBL)-type phenotype. We both ( bla CMY-2 ) ESBL-type TEM genes Plasmids nine transferred recipient strain J53 through conjugation. Transferable plasmids the also into lab-made sodium azide-resistant The class 1 integrase gene, int1 , was detected on two Further investigation integron cassette regions revealed presence aadA encoding streptomycin 3’’-adenylyltransferase, aadA1a / aadA2 aminoglycoside 3’’-O-adenyltransferase, putative adenylyltransferase gene. This study provides some insight potential horizontal events resistance between production.