Genetic analysis of Aphaniidae Hoedeman, 1949 (Teleostei: Cyprinodontiformes) family in Anatolia

We tested the mitochondrial DNA cytochrome b gene-based (1065 bp.) phylogeny and genetic diversity of tooth-carp fish (Aphaniidae Hoedeman, 1949 family), many which are endemic, with a very high species in Anatolia. A total 107 individuals were studied from 29 stations belonging to 19 Aphaniid species. For Cytb gene, 44 haplotypes identified, all species-specific. The phylogenetic relationships generated by neighbor joining, maximum likelihood parsimony methods fully compatible each other. generally well supported tree results distance structure divided into four lineages corresponding genera (Anatolichthys, Paraphanius, Aphanius, Kosswigichthys). between these indicated significant value ranging 16.6% 23.1%. Genetic interspecies distances 1.9% 24.52% (between A. villwocki P. mentoides), except for two groups (A. fontinalis – sureyanus, 0.13% maeandricus irregularis, 0.57%). Our also agree previous studies Anatolian Aphaniidae family, showed diversification pattern shaped Pliocene orogenic events. present indicate that gene sequences effective identification analysis.