Generation of a Chinese Hamster Ovary Cell Line Producing Recombinant Human Glucocerebrosidase
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چکیده
منابع مشابه
Generation of a Chinese Hamster Ovary Cell Line Producing Recombinant Human Glucocerebrosidase
Impaired activity of the lysosomal enzyme glucocerebrosidase (GCR) results in the inherited metabolic disorder known as Gaucher disease. Current treatment consists of enzyme replacement therapy by administration of exogenous GCR. Although effective, it is exceptionally expensive, and patients worldwide have a limited access to this medicine. In Brazil, the public healthcare system provides the ...
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Propranolol (PL), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. The present study was concerned with assessing the cytogenetic effects of this drug on Chinese hamster ovary (CHO) cell line. MTT assay was then carried out to determine the cytotoxicity index (IC50) of the drug. The IC50 value of PL was 0.43±0.02 mM. To investigate the clastogenic effect...
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Background: The critical role of interleukin-7 (IL-7) in homeostatic proliferation and T cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. Methods: In the present study we expressed recombinant human interleukin-7 (rhIL-7) in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected ...
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propranolol (pl), a non-selective beta-blocker, is a cardiovascular drug widely used to treat hypertension. the present study was concerned with assessing the cytogenetic effects of this drug on chinese hamster ovary (cho) cell line. mtt assay was then carried out to determine the cytotoxicity index (ic50) of the drug. the ic50 value of pl was 0.43±0.02 mm. to investigate the clastogenic effect...
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ژورنال
عنوان ژورنال: Journal of Biomedicine and Biotechnology
سال: 2012
ISSN: 1110-7243,1110-7251
DOI: 10.1155/2012/875383