Generation and characterization of chicken egg yolk antibodies (IgY) against TNFR1
نویسندگان
چکیده
منابع مشابه
GENERATION AND PHYSICOCHEMICAL CHARACTERIZATION OF CHICKEN EGG YOLK ANTIBODIES (IgY) AGAINST Salmonella typhimurium
Salmonellosis is one of the most important bacterial diseases with economic concern to all phases of the poultry. The study was aimed to generate chicken IgY against Salmonella typhimurium and its physicochemical characterization to unbolt the obstacles for the usage of IgY as therapeutic agent in Poultry. Salmonella typhimurium whole cell antigen was prepared and immunized in white leghorn chi...
متن کاملPurification and Characterization of Chicken Egg Yolk Antibodies (IgY) Against Mastitis Causing Klebsiella pneumoniae
White leghorn hens were immunized intramuscularly with formalin killed Klebsiella pneumoniae cells to generate Klebsiella pneumoniae antibodies (IgY) in egg yolk. Booster injections of increasing concentrations of antigen were given to raise the antibody level in egg yolk. Amount of protein varied from 0.87 7.4mg/ml in egg yolk throughout the immunization period. The antibodies were partially p...
متن کاملGeneration of Egg Yolk Antibodies in Chicken ( IgY ) Against Influenza M 2 ( M 2 e ) Protein
Chicken egg yolk immunoglobulins (IgY) from immunized hens have considerable advantages for the production of polyclonal antibodies. It reacts with more epitopes and avoids the interference in immunological assays. Regarding the IgY aptitudes, this study was conducted to produce chicken IgY specific for influenza M2 (M2e) protein. Fusion construct harboring C-terminal of bovine heat shock prote...
متن کاملSpecific Chicken Egg Yolk Antibodies against Enterotoxigenic Escherichia coli K99 in Serum and Egg Yolk of Immunized Laying Hens
Enterotoxigenic Escherichia coli K99 is one of the dominant pathogens associated with diarrhea of calves. Immunoglobulin Y (IgY), has been used as an inexpensive alternative to antibiotics for the prevention and therapy of several bacterial infections. The study aimed to prepare IgY antibodies against E. coli K99 and to investigate its in vitro effectiveness. E. c...
متن کاملProduction, characterization and application of monoclonal antibodies against chicken IgY
Monoclonal antibodies (Mabs) were produced against chicken IgY purified from egg yolks. Purity of the IgY was established using immunoelectrophoresis and reducing and non-reducing polyacrylamide gel electrophoresis. Five stable hybrid clones were characterized and Mabs, all belonging to IgG1 isotype, were found to bind to the IgY heavy chain in Western blots. These clones did not cross-react wi...
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ژورنال
عنوان ژورنال: Bratislava Medical Journal
سال: 2015
ISSN: 1336-0345
DOI: 10.4149/bll_2015_059