Generation and analysis of 280,000 human expressed sequence tags.
نویسندگان
چکیده
منابع مشابه
Generation and analysis of expressed sequence tags from Botrytis cinerea.
Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the uni...
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Expressed sequence tags (ESTs) present a special set of problems for bioinformatic analysis. They are partial and error-prone, and large datasets can have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated "pipeline" of tools that take EST data from sequence trace to database submission. These tools also can be used to ...
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Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...
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We have generated 2771 expressed sequence tags (ESTs) from two cDNA libraries of Trypanosoma cruzi CL-Brener. The libraries were constructed from trypomastigote and amastigotes, using a spliced leader primer to synthesize the cDNA second strand, thus selecting for full-length cDNAs. Since the libraries were not normalized nor pre-screened, we compared the representation of transcripts between t...
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In an effort to identify novel genes that are expressed differentially in an infant thymus, we constructed an oligo-d(T) primed cDNA library from a human infant thymus followed by single-run partial sequencing to generate expressed sequence tags (ESTs). Characterization of more than 1400 sequences enabled us to convert human thymus transcripts into 1223 useful ESTs. These ESTs consisted of 613 ...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1996
ISSN: 1088-9051
DOI: 10.1101/gr.6.9.807