Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment
نویسندگان
چکیده
Abstract Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis usually heterozygous during the early generations. However, must be fixed prior to phenotyping field trials, which requires additional rounds of self-pollination. Microspore culture is an effective method produce double-haploid (DH) plants that homozygotes. In this study, we conducted ethyl methanesulfonate (EMS)-induced microspore cultures barley ( Hordeum vulgare ) cultivar ‘Hua30’ landrace ‘HTX’. The EMS concentrations were negatively correlated with efficiency callus induction frequency mutant plant regeneration. two genotypes showed different regeneration efficiencies. phenotypic variation regenerated M 1 presence genome-wide nucleotide mutations, revealed whole-genome sequencing, highlight utility EMS-induced isolated developing DH mutants. Genome-wide analysis mutation in a considerable proportion resulted from (somaclonal variation) rather than mutagenesis. addition producing population 1972 homozygous lines available future study lays foundation optimizing richness (mainly fine-tuning mutagen dosage).
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The effect of mixed mutagen exposures on the rate and type of induced mutants was studied in the L5178Y/TK+/-----TK-/- mouse lymphoma cell mutagenicity assay. In this assay, exposure to ethyl methanesulfonate (EMS) results in more mutants that form large colonies than small colonies. Exposure to methyl methanesulfonate (MMS) results in more mutants that form small colonies than large colonies. ...
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ژورنال
عنوان ژورنال: aBIOTECH
سال: 2023
ISSN: ['2096-6326', '2662-1738']
DOI: https://doi.org/10.1007/s42994-023-00108-6