Gene2Oligo: oligonucleotide design for in vitro gene synthesis
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چکیده
منابع مشابه
Gene2Oligo: oligonucleotide design for in vitro gene synthesis
There is substantial interest in implementing a bioinformatics tool that allows the design of oligonucleotides to support the development of in vitro gene synthesis. Current protocols to make long synthetic DNA molecules rely on the in vitro assembly of a set of short oligonucleotides, either by ligase chain reaction (LCR) or by assembly PCR. Ideally, such oligonucleotides should represent both...
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Herein we present TmPrime, a computer program to design oligonucleotide sets for gene assembly by both ligase chain reaction (LCR) and polymerase chain reaction (PCR). TmPrime offers much flexibility with no constraints on the gene and oligonucleotide lengths. The program divides the long input DNA sequence based on the input desired melting temperature, and dynamically optimizes the length of ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkh401