Gene Transfer of Connexin43 into Skeletal Muscle
نویسندگان
چکیده
منابع مشابه
Gene transfer of connexin43 into skeletal muscle.
Cellular cardiomyoplasty using skeletal myoblasts may be beneficial for infarct repair. One drawback to skeletal muscle cells is their lack of gap junction expression after differentiation, thus preventing electrical coupling to host cardiomyocytes. We sought to overexpress the gap junction protein connexin43 (Cx43) in differentiated skeletal myotubes, using retroviral, adenoviral, and plasmid-...
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Current gene therapy strategies using adenoviral vectors to target the lung or liver have been complicated by an acute inflammatory response that can result in loss of transgene expression as well as tissue injury and necrosis. Skeletal muscle comprises 4 0 % oftotal body weight; it possesses a high density, accessible capillary network that is resistant to injury and thus m a y be a logical ta...
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For most experimental and therapeutic applications of gene transfer, regulation of the timing and level of gene expression is preferable to constitutive gene expression. Among the systems that have been developed for pharmacologically controlled gene expression in mammalian cells, the bacterial tetracycline (tet)-responsive system has the advantage that it is dependent on a drug (tet) that is b...
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Gene delivery to adult skeletal muscle is a promising strategy for myofiber signal transduction studies and for the treatment of muscle disorders. Among the nonviral techniques for gene transfer in vivo, electric field applied to the direct injection of plasmid DNA into muscle is safe, inexpensive and simple. However, present DNA delivery technologies should be improved with regard to the level...
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Gene delivery to skeletal muscle is a promising strategy for the treatment of muscle disorders and for the systemic secretion of therapeutic proteins. However, present DNA delivery technologies have to be improved with regard to both the level of expression and interindividual variability. We report very efficient plasmid DNA transfer in muscle fibers by using square-wave electric pulses of low...
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ژورنال
عنوان ژورنال: Human Gene Therapy
سال: 2004
ISSN: 1043-0342,1557-7422
DOI: 10.1089/1043034041361253