منابع مشابه
Gene Transfer - Induced Helper T Cells 2189
Rev MlO is a ^ans-dominant negative inhibitor of HIV replication. Hence, stable transduction of CD4"'" T cells with Rev M l O represents a novel gene therapy aimed at inhibiting H I V replication within these cells, thereby slowing the progression of AIDS. However, the immune system m a y recognize Rev M l O as foreign and target transduced cells for elimination. In the current study, mice were...
متن کامل729-electric Gene Transfer into Culture Cells *
The electric field method of gene transfer via electroporation of cell membranes is gaining importance in cellular and molecular biology. In particular the electroporation technique provides the low copy number transfection required fqr genetic studies. As demonstrated with mouse lyoma cells, with lymphoid cell lines and with plant protoplast, the electric method has appreciable advantages comp...
متن کاملI. Gene Transfer into Hematopoietic Stem Cells
This article reviews 1) the use of gene transfer methods to genetically manipulate hematopoietic stem cell targets, 2) recent advances in technology that are addressing problems that have prevented widespread successful translation of gene transfer approaches for the cure of disease, and 3) recent regulatory issues related to human gene therapy trials. In Section I, Dr. Nienhuis describes the u...
متن کاملUltrasound-mediated gene transfer into neuronal cells.
A new field of gene transfer is emerging as a simple, effective means to drive the expression foreign genes in cells: ultrasound-mediated gene transfer or sonoporation. We report here that sonoporation is an effective means of gene transfer for cultured neurons, a cell type that has been difficult to transfect. Neuronal cell types that are effectively sonoporated include chick retinal neurons, ...
متن کامل0231 - Gene Gun-mediated Gene Transfer to Intervertebral Disc Cells
INTRODUCTION: Gene therapy using retroviral and, more recently, adenoviral vectors is gaining interest in orthopedics. Particle-mediated gene transfer with a gene gun is a novel non-viral alternative that carries great promise [1]. The basic principle of particle-mediated gene transfer is to use high velocity DNA-coated gold particles that are accelerated by high pressure helium gas to penetrat...
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ژورنال
عنوان ژورنال: Nature
سال: 2015
ISSN: 0028-0836,1476-4687
DOI: 10.1038/nature15205