Gene replacement in Staphylococcus carnosus and Staphylococcus xylosus
نویسندگان
چکیده
منابع مشابه
Surface display of the cholera toxin B subunit on Staphylococcus xylosus and Staphylococcus carnosus.
The heterologous surface expression of the cholera toxin B subunit (CTB) from Vibro cholerae in two staphylococcal species, Staphylococcus xylosus and Staphylococcus carnosus, has been investigated. The gene encoding native CTB (103 amino acids) was introduced into gene constructs encoding chimeric receptors designed to be translocated and anchored on the outer cell surface of the staphylococci...
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Starter cultures, by their biochemical activity, cause changes to the pH of the meat, speed up the development of colour, consistency, fl avour, aroma, and in general bring about the formation of the characteristic qualities of the product. Alongside their desired eff ect, some microbe cultures may cause the product to spoil; it is also possible to fi nd microorganisms which may be harmful for ...
متن کاملGenomic diversity in Staphylococcus xylosus.
Staphylococcus xylosus is a commensal of the skin of humans and animals and a ubiquitous bacterium naturally present in food. It is one of the major starter cultures used for meat fermentation, but a few strains could potentially be hazardous and are related to animal opportunistic infections. To better understand the genetic diversity of S. xylosus intraspecies, suppressive and subtractive hyb...
متن کاملComplete nucleotide sequence of the lipase gene from Staphylococcus hyicus cloned in Staphylococcus carnosus.
The lipase gene from Staphylococcus hyicus subsp. hyicus was cloned in Staphylococcus carnosus and Escherichia coli. In both host organisms the lipase gene is expressed and the enzyme is released to the medium. The cloned DNA insert is 2.5 kb in length and DNA sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence. The open rea...
متن کاملCharacterization of a sucrase gene from Staphylococcus xylosus.
The Staphylococcus xylosus gene scrB, encoding a sucrase, has been isolated from a genomic library of S. xylosus constructed in Escherichia coli. The gene was detected by its ability to confer utilization of the glucose and fructose residues of raffinose in an E. coli strain that is not able to metabolize galactose. It was found to reside within a 1.8-kb DNA fragment, the nucleotide sequence of...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2006
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1997.tb10387.x