Gene Replacement in Gram-Negative Bacteria: the pMAKSAC Vectors

Gene replacement in gram-negative bacteria: the pMAKSAC vectors.

1.Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580. 2.Ishii, T.M., P. Zerr, X.M. Xia, C.T. Bond, J. Maylie and J.P. Adelman. 1998. Site-directed mutagenesis. Methods Enzymol. 293:53-71. 3.Kirsch, R.D. and E. Joly. 1998. An improved PCR-mutagenesis strategy for two-site mutagenesis or sequence swapping between related genes. Nucleic Acids R...

Antibiotic Resistance Pattern of Gram-Negative Bacteria in Gorgan

Abstract Background and Objective: The excessive use of broad-spectrum antibiotics will lead to drug resistance of microorganism and specially nosocomial organisms. Because of high incidence of antibiotic resistance in hospitals, we aimed to study antibiotic resistance to gram negative bacteria. Material and Methods: This cross-sectional study was conducted on the data of biological sampl...

Cell Envelope Structure and Functions in Gram-negative Bacteria

New Mobilizable Vectors Suitable for Gene Replacement in Gram-Negative Bacteria and Their Use in Mapping of the 39 End of the Xanthomonas campestris pv. campestris gum Operon

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3* end of the Xanthomonas campestris gum operon, involved in biosynthesi...

New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon.

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesi...