Gene markers for grain polyphenol oxidase activity in common wheat
نویسندگان
چکیده
منابع مشابه
An Improved Whole-Seed Assay for Screening Wheat Germplasm for Polyphenol Oxidase Activity
portant for consumer acceptance (Moss, 1971; Miskelly, 1984, 1996), while low L* values indicate undesirable Polyphenol oxidase (PPO) causes darkening and discoloration of discoloration or darkening of noodles. wheat (Triticum aestivum L.) foods such as noodles. Consequently, a simple, nondestructive, quantitative assay for determining PPO on There is a need for a simple, quantitative assay for...
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Sequencing of the genome of Ralstonia solanacearum revealed several genes that putatively code for polyphenol oxidases (PPOs). To study the actual expression of these genes, we looked for and detected all kinds of PPO activities, including laccase, cresolase, and catechol oxidase activities, in cellular extracts of this microorganism. The conditions for the PPO assays were optimized for the phe...
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The electrochemistry of banana tissues on a carbon paste electrode modified with multi-walled carbon nanotubes (MWCNTs) is presented. Cyclic voltammetry is applied to investigate the direct electrochemistry of banana tissues i.e. a source of polyphenol oxidase (PPO). A redox couple with an anodic and counterpart cathodic peak is obtained. The influence of various parameters such as pH,...
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Polyphenol oxidase (PPO; EC 1.10.3.2) was isolated from wheat bran by a procedure that included ammonium sulfate fractionation, batch adsorption by DEAE-cellulofine, CM-cellulofine column chromatography, DEAE-cellulofine column chromatography, preparative isoelectric focusing, adsorption on the membrane of a Vivapure Q Maxi H spin column, and heat treatment. These procedures led to 150-fold pur...
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Polyphenol oxidase (PPO) activity varied in differentiation from hyphae to rhizomorph and croute in A. mellea. In undifferentiated hyphae there was not any PPO activity, while croute showed the highest activity. Electrophoresis in the presence of SDS allows the detection of intensive enzyme forms of PPO, and can also convert slower migrating enzyme forms to faster ones. Some fluorescent co...
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ژورنال
عنوان ژورنال: Molecular Breeding
سال: 2008
ISSN: 1380-3743,1572-9788
DOI: 10.1007/s11032-008-9223-1