Gene expression signatures associated with chronic endometritis revealed by RNA sequencing

Introduction Chronic endometritis (CE) is a persistent inflammatory condition of the endometrium characterized by infiltration plasma cells in endometrial stroma. CD138 immunohistochemistry considered to improve CE diagnosis rate. Methods Using number CD138-positive equal or greater than five as diagnostic criterion for CE, we identified 24 and 33 non-CE cases among women with infertility. We conducted RNA-sequencing analysis these 57 total an attempt elucidate molecular pathogenesis search new biomarkers CE. Results Discussion By comparing groups, 20 genes upregulated endometria patients, including 12 immunoglobulin-related eight non-immunoglobulin differentially expressed genes. The were MUC5AC , LTF CAPN9 MESP1 ACSM1 TVP23A ALOX15 MZB1 . analyzing samples proliferative secretory phases menstrual cycle separately, also four additional endometria: CCDC13 phase, OVGP1 MTUS2 CLIC6 phase. Although may serve novel markers many them only limited showing extremely high near over one hundred. Exceptionally, was majority regardless -positive cells. upregulation reflect pathophysiology cell-type cell-types intrinsic rather accumulation Our data, consisting clinical transcriptomic information cases, helped us identify gene expression signatures associated