Gene Cloning and Properties of the RND-Type Multidrug Efflux Pumps MexPQ-OpmE and MexMN-OprM fromPseudomonas aeruginosa
نویسندگان
چکیده
منابع مشابه
Tripartite assembly of RND multidrug efflux pumps
Tripartite multidrug efflux systems of Gram-negative bacteria are composed of an inner membrane transporter, an outer membrane channel and a periplasmic adaptor protein. They are assumed to form ducts inside the periplasm facilitating drug exit across the outer membrane. Here we present the reconstitution of native Pseudomonas aeruginosa MexAB-OprM and Escherichia coli AcrAB-TolC tripartite Res...
متن کاملCharacterization of the RND-type multidrug efflux pump MexAB-OprM of the plant pathogen Pseudomonas syringae.
In gram-negative bacteria, transporters belonging to the RND family are the transporters most relevant for resistance to antimicrobial compounds. In Pseudomonas aeruginosa, a clinically important pathogen, the RND-type pump MexAB-OprM has been recognized as one of the major multidrug efflux systems. Here, homologues of MexAB-OprM in the plant pathogens Pseudomonas syringae pv. phaseolicola 1448...
متن کاملA new member of the tripartite multidrug efflux pumps, MexVW-OprM, in Pseudomonas aeruginosa.
OBJECTIVES Multidrug efflux pumps are thought to be involved in mediating multidrug resistance in Pseudomonas aeruginosa. Here we aim to characterize hitherto uncharacterized multidrug efflux pumps from P. aeruginosa. MATERIALS AND METHODS We isolated a mutant, YM442, which showed elevated resistance to several antimicrobial agents from P. aeruginosa YM44 lacking four major multidrug efflux p...
متن کاملMolecular Dynamics Computer Simulations of Multidrug RND Efflux Pumps
Over-expression of multidrug efflux pumps of the Resistance Nodulation Division (RND) protein super family counts among the main causes for microbial resistance against pharmaceuticals. Understanding the molecular basis of this process is one of the major challenges of modern biomedical research, involving a broad range of experimental and computational techniques. Here we review the current st...
متن کاملSubstrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa.
To find the exact substrate specificities of three species of tripartite efflux systems of Pseudomonas aeruginosa, MexAB-OprM, MexCD-OprJ, and MexXY-OprM, we constructed a series of isogenic mutants, each of which constitutively overproduced one of the three efflux systems and lacked the other two, and their isogenic mutants, which lacked all these systems. Comparison of the susceptibilities of...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 2005
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.2005.tb03696.x