Gene Amplification in Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
amplification, sequencing and cloning of iranian native bacillus subtilis alpha-amylase gene in saccharomyces cerevisiae
conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...
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1 Sporulation in Bacillus subtilis serves as a paradigm for the development of two different cell types (mother cell and prespore) from a single cell. The mechanism by which the two different developmental programs are initiated has been much studied but is not well understood. With the help of existing and new experimental results, a mathematical model has been developed that reproduces all pu...
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To evaluate the role of wild promoter of L-phenylalanine dehydrogenase (PheDH) gene, referred to as pdh, from Bacillus sphaericus in expression, cloning of pdh gene in Bacillus subtilis was performed. The whole pdh gene was cloned in pHY300PLK shuttle vector and amplified, construct (pHYDH) then transformed in B. subtilis ISW1214 and E. coli JM109. The pdh endogenous promoter presented no effec...
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A recombinant plasmid was constructed by ligation of chromosomal DNA from a sulfanilamide-resistant strain of Bacillus subtilis to the plasmid vector pUB110 which specifies neomycin resistance. Recombinant molecules generated in vitro were introduced into a B. subtilis recipient strain which carried the recE4 mutation, and selection was for neomycin-sulfanilamide-resistant transformants. A sing...
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Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a dir...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1984
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-130-7-1613