Gelatin-hydroxyapatite Fibrous Nanocomposite for Regenerative Dentistry and bone Tissue Engineering

Aims: This study aimed to prepare and physicochemically evaluate as well assess the cytotoxicity stimulation of early osteogenic differentiation dental pulp stem cells gelatin-hydroxyapatite (Gel-HA) fibrous nanocomposite scaffold. Background: Recently, electrospinning approach in nanotechnology has been considered due its application preparation biomimetic nanofibers for tissue engineering. Objective: The main objective this was Gel-HA regenerative dentistry bone engineering material. Methods: nano-scaffold prepared via method. Then, physicochemical properties (particle size, surface charge, morphology, hydrophilicity, specific area, crystalline state characterization functional groups) proliferative effects nano-scaffolds on were assessed. alkaline phosphatase activity assessed evaluation cells. Results: had a negative charge (-30 mv±1.3), mono-dispersed nano-scale diameter (98 nm±1.2), uniform morphology without any bead (structural defects). nanofibrous scaffold showed increased hydrophobicity compared gelatin nanofibers. Based Brunauer-Emmett-Teller analysis, pore volume decreased nano-fibers effect significantly (P<0.05). Conclusion: can be potential candidates dentistry. Other: could material regeneration near future.