Gel electrophoresis and isoelectric focusing of proteins
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چکیده
منابع مشابه
Two-dimensional gel electrophoresis, isoelectric focusing and agarose gel electrophoresis in the diagnosis of multiple sclerosis.
Two dimensional gel electrophoresis (2-DE), isoelectric focusing (IEF) and agarose gel electrophoresis (AGE) were used to examine cerebrospinal fluid (CSF) and sera from 22 patients with confirmed multiple sclerosis, 11 patients with probable multiple sclerosis and 20 control patients with non-inflammatory neurological diseases of the central nervous system (CNS). All of the 22 patients with co...
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Two-dimensional gel electrophoresis (2DE) is a well-developed, high-resolution technology to separate complex mixtures of proteins. This technique has been widely used for the past four decades, and has played a vital part in the developmental history of proteomics. The advantages and limitations of 2DE in proteomics research have been reviewed (e.g., Thelen and Peck, 2007; Oliveira et al., 201...
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While two-dimensional electrophoresis (2-DE) of proteins, as we know it, was first described nearly four decades ago (O’Farrell, 1975), it continues to be a critical component of comprehensive proteomics analysis today. Continuing refinements of 2-DE, particularly the development of immobilized pH gradients (Righetti, 1990), new chaotropes (Vecchio et al. 1984, Rabilloud 1998) and detergents (S...
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A preparative isoelectric focusing (IEF) method was applied to separate skim milk proteins using the Rotofor device in a pH 3-10 gradient containing 4 M urealI % triton X-I 00. Each ofthe 20 fractions obtainedfrom the Rotofor device was then analyzed by polyacrylamide gel electrophoresis (PAGE). Both ureaPAGE and SDS-PAGE were used to separate purified caseins and skim milk resulting in compara...
متن کاملIsoelectric focusing of proteins in rewetted ultrathin polyacrylamide gel layers.
Ultrathin layers of polyacrylamide gel bound to glass can be washed, air-dried, and stored for at least 1 year before rewetting in ampholyte solutions for isoelectric focusing. Short-term drying affects neither fluorescent banding of the ampholytes (not evident in conventional gels) nor resolution of complex protein mixtures while prolonged storage seems to have no deleterious effects. Layers a...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1986
ISSN: 0014-5793
DOI: 10.1016/0014-5793(86)80083-7