GC-MS BIOPROSPECTING OF PHYTOCHEMICALS IN THE ETHANOLIC EXTRACT OF FRESH Mangifera indica LEAVES

A structural analysis of phytochemical constituents present in the ethanolic extract Mangifera indica fresh leaves were investigated to assert presence bioactive compounds corresponding reported antioxidant, antibacterial, antihyperglycemic, antidiabetic and anthelmintics properties plant. Extraction was carried out on homogenised using conventional soxhlet apparatus, with ethanol chosen selectively polar higher terpenes hydrocarbons. The (MIFL) separated quantified by a Gas Liquid Chromatography, while identification elucidation phytochemicals obtained Mass spectrometer producing spectra lines high accuracy which molecular ion not lost. GC-MS indicated total 20 identified MIFL comprising; Five sesquiterpenes (1H-cycloprope-azulene, caryophyllene, humulene, 2methylene, 6 methyl, 9 propenyl - decahydro-Naphthalene 5 isopropenyl,3,8 dimethyl octahydro-azulene), five hydrocarbons (consisting E-15 heptadecanal, 3-eicosene, 1-docosene, tritetracontane octacosane), four-4 fatty acids esters (hexadecanoic acid methyl ester, dibutyl phthalate, bis (2-ethylhexyl N-(2,5 dimethyl-fumaric heptyl ester), , four alkaloids two plant steroids (composed beta amyrin 9,19-cyclolanost-24-en-3-ol). detected showed consistent m/e ratio 204 varying base peaks accordance sesquiterpene formula C15H24. part whole characterised indicates following: 22.22% fraction steroids, 25.85% identified, 18.28% found 26.12% di-2 ethylhexyl phthalate having highest peak area representing 16.72% total. Reported biological activities for are stated as follows: antibacterial activity attributed β-caryophyllene, di-(2-ethylhexyl) DEHP. [ ], Antioxidant anticancer exhibited β-caryophyllene β-amyrin steroid elucidated, responsible antifungal antihyperglycemic activity.