GATMD: -Aminobutyric Acid Transporter Mutagenesis Database
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چکیده
منابع مشابه
GATMD: γ-Aminobutyric Acid Transporter Mutagenesis Database
Since the cloning of the first γ-aminobutyric acid (GABA) transporter (GAT1; SLC6A1) from rat brain in 1990, more than 50 published studies have provided structure-function information on investigator-designed rat and mouse GAT1 mutants. To date, more than 200 of 599 GAT1 residues have been subjected to mutagenesis experiments by substitution with different amino acids, and the resulting transp...
متن کاملy-aminobutyric acid
An enzyme which catalyses dehydrogenation of y-aminobutyraldehyde (ABAL) to y-aminobutyric acid (GABA) was purified to homogeneity from rat brain tissues by using DEAE-cellulose and affinity chromatography on 5'AMP-Sepharose, phosphocellulose and Blue Agarose, followed by gel filtration. Such an enzyme was first purified from mammalian brain tissues, and was identified as an isoenzyme of aldehy...
متن کاملAtGAT1, a High Affinity Transporter for -Aminobutyric Acid in Arabidopsis thaliana*
Functional characterization of Arabidopsis thaliana GAT1 in heterologous expression systems, i.e. Saccharomyces cerevisiae and Xenopus laevis oocytes, revealed that AtGAT1 (At1g08230) codes for an H -driven, high affinity -aminobutyric acid (GABA) transporter. In addition to GABA, other -aminofatty acids and butylamine are recognized. In contrast to the most closely related proteins of the prol...
متن کاملA Binding Mode Hypothesis of Tiagabine Confirms Liothyronine Effect on γ-Aminobutyric Acid Transporter 1 (GAT1)
Elevating GABA levels in the synaptic cleft by inhibiting its reuptake carrier GAT1 is an established approach for the treatment of CNS disorders like epilepsy. With the increasing availability of crystal structures of transmembrane transporters, structure-based approaches to elucidate the molecular basis of ligand-transporter interaction also become feasible. Experimental data guided docking o...
متن کاملγ-Aminobutyric acid transporter-mediated current from bipolar cells in tiger salamander retinal slices
About 10% of bipolar cells in salamander retina synthesize and take up gamma-aminobutyric acid (GABA), and may use GABA as a neurotransmitter. As GABA uptake is electrogenic, bipolar cells expressing GABA transporters (GATs) should give transport current (IGAT) to extracellular GABA. Using whole-cell patch recording, 28 bipolar cells responded to 30-200 microM GABA puffed to the axon terminals ...
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ژورنال
عنوان ژورنال: Database
سال: 2010
ISSN: 1758-0463
DOI: 10.1093/database/baq028