Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging
نویسندگان
چکیده
منابع مشابه
Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents.
We describe here a multiplexed protein quantitation strategy that provides relative and absolute measurements of proteins in complex mixtures. At the core of this methodology is a multiplexed set of isobaric reagents that yield amine-derivatized peptides. The derivatized peptides are indistinguishable in MS, but exhibit intense low-mass MS/MS signature ions that support quantitation. In this st...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2011
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.1716