Gallic acid improves the viability and mitochondrial membrane potential of post-thawed goat buck semen

Abstract The aim of this study was to determine the effects gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli bucks were used at their breeding season, and ejaculates collected by an electroejaculator. Mixed semen divided into following four groups: control (0 mM), low (L; 1 medium (M; 2 high (H; 4 mM) concentration GA. All groups frozen thawed in a water bath for spermatological evaluation. lowest motility observed group (47.60 ± 5.70%) ( P < 0.05), while highest viability (62.45 1.68%), plasma membrane acrosome integrity (44.81 4.57%), mitochondrial potential (35.96 2.50%) GA 0.05). Also, hypo-osmotic swelling test (HOS +) value found 4.82%) In conclusion, supplementing (1 Tris-based extender had positive effect parameters after freeze-thawing semen. Further studies should be continued other species with different doses combinations using commercial and/or homemade extenders.