Galactose Transport in Escherichia coli and the Galactose-Binding Protein
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منابع مشابه
Galactose-Binding Protein by the Cell Cycle of Escherichia coli
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The galactose-binding protein, a necessary component of a bacterical transport system, is shown by three independent methods to undergo a conformational change upon binding of substrate. 1. A Lineweaver-Burk plot of the binding data obtained by equilibrium dialysis at a protein concentration of 0.4 mg per ml shows heterogenous behavior. Extrapolation of the data at low galactose concentrations ...
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The beta-methyl-galactoside- and galactose-specific transport systems of Escherichia coli were shown by experiments involving inhibitors and the use of an adenosine triphosphatase mutant strain to utilize adenosine 5'-triphosphate or a related compound to drive active transport. These systems were shown to be unable to use the activated-membrane state. The galactose-specific transport system wa...
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The intracellular concentration of nonmetabolixable compounds in bacteria can be depicted as the result of a difference between the rate of entry and the rate of exit (l-3). The entry mechanism, originally envisioned as the only active process in the system, has been termed “permease” because of its enzymic behavior (1). The exit mechanism, previously regarded as passive diffusion, is now also ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1972
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1972.tb01922.x