G1 Ganglioside β-Galactosidase A

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Cloning, High-level Expression and Characterization of a β-Galactosidase from Bacillus subtilis G1

A gene (lacA) coding for β-galactosidase from Bacillus subtilis strain G1 was cloned, expressed in E. coli and the β-galactosidase LacA was purified and characterized. The lacA gene consisted of an open reading frame of 2061 nucleotides, encoding a 686-aa protein, with a predicted molecular mass of 79 kDa and pI 6.16. The β-galactosidase showed an identity of 97.8% with the corresponding amino ...

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β-galactosidase stability at high substrate concentrations

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1974

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(19)42060-7