Further RFLPs at the human tyrosine hydroxylase locus

Two RFLPs at the TNP1 locus.

Source/Description: The Human Transition Protein 1 gene (TNP1) probe (1) used was a 700 bp genomic DNA sequence cloned into the Smal site of pUC8 and was supplied by W.Engel. Polymorphisms: TNP1 identifies a two allele polymorphism with TaqI: Al = 9.6 kb; A2 = 8.4 kb. Frequency: Analysis of 20 unrelated individuals yielded frequencies: Al = 0.23; A2 = 0.77. Rsal in a screen of 10-20 unrelated i...

RFLPs at the D19S19 locus of human chromosome 19 linked to myotonic dystrophy (DM)

SOURCE/DESCRIPTION: Probe LDR152-a 1.68 Kb single copy Hind III fragment isolated from a chromosome 19 bivariate flow sorted enriched Charon 21A library and subcloned into SP64. CHROMOSOMAL LOCALISATION: 1 9cent-1 9q 1 • 3 by somatic cell hybrid and linkage data. POLYMORPHISMS: Recognises two, two allele polymorphisms when U3ed to probe human genomic DNA digested with P3t I and Msp I.

Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain.

In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Immunoreactive TH on PVDF membranes was identified with optimized concentrations...

RFLPs at the PAH (phenylalanine hydroxylase) gene in the Japanese population.

Source and Description of Clone: phPAH247, a 2.4 kb EcoSl fragment subcloned into pBR322 from a human liver cDNA library (1).

Phenylalanine Hydroxylase, Tyrosine Hydroxylase, and Tryptophan Hydroxylase