Further heterogeneity demonstrated for serum creatine kinase isoenzyme MM.
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چکیده
منابع مشابه
Further heterogeneity demonstrated for serum creatine kinase isoenzyme MM.
Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes were also demonstrated in patients undergoing coronary-bypass surgery. The evolution of two CK-MB sub-bands was studied in both cases. We found that three electrophoretic bands (CK-MM, pI 7.10; M...
متن کاملHeterogeneity of serum creatine kinase isoenzyme MM in myocardial infarction: clinical significance and post-synthetic conversion of "abnormal" sub-bands.
We investigated the "abnormal" sub-bands of serum creatine kinase (CK; EC 2.7.3.2) isoenzyme MM in acute myocardial infarction (AMI), using isoelectric focusing in polyacrylamide gels, and compared the patterns with those for non-AMI patients and for athletes with increased CK. The "abnormal" sub-bands a (pI 7.55), b (pI 7.35), c (pI 7.25), d (pI 7.05), e (pI 6.85), f (pI 6.72), g (pI 6.50), h ...
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The routine determination of creatine kinase (CK; EC 2.7.3.2) isoenzymes has recently gained acceptance as a diagnostic tool in laboratory medicine (13). Electrophoreticaily three distinct CK isoenzymes can be separated (4, 5). These dimeric bands have been identified throughout the literature as MM, MB, and BB, and originate mainly from skeletal muscle, heart, and brain tissues, respectively. ...
متن کاملSerum creatine kinase MM isoenzyme sub-bands after acute myocardial infarction in man.
Using an isoelectric-focusing (IEF) method developed to quantitate MM isoenzyme-creatine kinase (CK) sub-band activity, we identified a reproducible time-varying pattern of these sub-bands in the serum of eight patients with acute myocardial infarction (MI). Our observations are consistent with the view that MM3-CK (the M2-CK dimer, the pure gene product) is converted intravascularly to MM2-CK,...
متن کاملThe detection of creatine kinase isoenzyme CK1 in serum.
The brain isoenzyme of creatine kinase (CK) (EC 2.7.3.2) has been identified in the serum of 21 of 950 patients tested. Samples were received over a period of about 20 months. Analysis was performed by electrophoresis on agarose gel with isoenzyme bands visualized by fluorescence after enzyme coupled reactions producing NADH. Specialized substrate preparations were used to rule out the possibil...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1980
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/26.3.457