Functional Screen of the Fanconi Anemia Pathway in Cancer Cells by Fancd2 Immunoblot
نویسندگان
چکیده
منابع مشابه
Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein.
The Fanconi anemia (FA)-BRCA pathway is critical for the repair of DNA interstrand crosslinks (ICLs) and the maintenance of chromosome stability. A key step in FA-BRCA pathway activation is the covalent attachment of monoubiquitin to FANCD2 and FANCI. Monoubiquitinated FANCD2 and FANCI localize in chromatin-associated nuclear foci where they interact with several well-characterized DNA repair p...
متن کاملDefective FANCI Binding by a Fanconi Anemia-Related FANCD2 Mutant
FANCD2 is a product of one of the genes associated with Fanconi anemia (FA), a rare recessive disease characterized by bone marrow failure, skeletal malformations, developmental defects, and cancer predisposition. FANCD2 forms a complex with FANCI (ID complex) and is monoubiquitinated, which facilitates the downstream interstrand crosslink (ICL) repair steps, such as ICL unhooking and nucleolyt...
متن کاملRegulated interaction of the Fanconi anemia protein, FANCD2, with chromatin.
DNA damage activates the monoubiquitination of the Fanconi anemia (FA) protein, FANCD2, resulting in the assembly of FANCD2 nuclear foci. In the current study, we characterize structural features of FANCD2 required for this intranuclear translocation. We have previously identified 2 normal mRNA splice variants of FANCD2, one containing exon 44 sequence at the 3' end (FANCD2-44) and one containi...
متن کاملHistone H2AX and Fanconi anemia FANCD2 function in the same pathway to maintain chromosome stability.
Fanconi anemia (FA) is a chromosome fragility syndrome characterized by bone marrow failure and cancer susceptibility. The central FA protein FANCD2 is known to relocate to chromatin upon DNA damage in a poorly understood process. Here, we have induced subnuclear accumulation of DNA damage to prove that histone H2AX is a novel component of the FA/BRCA pathway in response to stalled replication ...
متن کاملAdvances in the understanding of Fanconi Anemia Complementation Group D2 Protein (FANCD2) in human cancer.
Fanconi anemia (FA) is a rare human genetic disease, resulting from dysfunction in any of 17 known complementation proteins: FANC-A, B, C, D1, D2, E, F, G, I, J, L, M, N, O, P, Q & S, and other unknowns. Besides the severe bone marrow failure, an extremely high incidence of cancer as well as many other clinic symptoms associated with FA patients, FA cells are known of insufficiency in homologou...
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ژورنال
عنوان ژورنال: Cancer Biology & Therapy
سال: 2004
ISSN: 1538-4047,1555-8576
DOI: 10.4161/cbt.3.6.844