Functional Reconstitution of a Prokaryotic K+ Channel
نویسندگان
چکیده
منابع مشابه
Functional Reconstitution of a Prokaryotic K+ Channel
SliK, a K+ channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted in liposomes. A concentrative 86Rb+ flux assay was used to assess the ion transport properties of SliK. SliK-mediated ionic flux shows strong selectivity for K+ over Na+ and is inhibited by micromolar concentrations of Ba2+, mirroring the basic permeation characteristic of eukaryotic K+ channels...
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SliK, a K 1 channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted in liposomes. A concentrative 86 Rb 1 flux assay was used to assess the ion transport properties of SliK. SliK-mediated ionic flux shows strong selectivity for K 1 over Na 1 and is inhibited by micromolar concentrations of Ba 2 1 , mirroring the basic permeation characteristic of eukaryotic K 1...
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ClC-type anion-selective channels are widespread throughout eukaryotic organisms. BLAST homology searches reveal that many microbial genomes also contain members of the ClC family. An Escherichia coli-derived ClC Cl(-) channel homologue, "EriC," the product of the yadQ gene, was overexpressed in E. coli and purified in milligram quantities in a single-step procedure. Reconstitution of purified ...
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KvLm is a prokaryotic voltage-gated K(+) (Kv) channel from Listeria monocytogenes. The sequence of the voltage-sensing module (transmembrane segments S1-S4) of KvLm is atypical in that it contains only three of the eight conserved charged residues known to be deterministic for voltage sensing in eukaryotic Kv's. In contrast, the pore module (PM), including the S4-S5 linker and cytoplasmic tail ...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1998
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.111.6.741