Functional characteristic of PC12 cells with reduced microsomal glutathione transferase 1.

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Functional characteristic of PC12 cells with reduced microsomal glutathione transferase 1.

Microsomal glutathione transferase 1 (MGST1) possesses glutathione transferase and peroxidase activities and is active in biotransformation of xenobiotics and in defense against oxidative stress. To assess MGST1 role in the development and functioning of PC12 cells, we constructed a cell line with reduced MGST1 (PC12_M). Real-time PCR and immunoblot assays showed MGST1 expression lowered to 60 ...

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Ligand Binding and Mechanism of Microsomal Glutathione Transferase 1

The homo-trimeric, membrane bound Microsomal Glutathione Transferase 1 (MGST1, EC. 2.5.1.18) belongs functionally to both the glutathione transferase family (GST) and the Membrane Associated Proteins in Eicosanoid and Glutathione Metabolism (MAPEG) superfamily. It is found in high amount in the liver, where it is localised to the endoplasmatic reticulum and the outer membrane of mitochondria. M...

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Glutathione transferases (GSTs) are often upregulated in tumors and have been suggested to play an important role in multiple drug resistance in cancer chemotherapy. As a consequence GST-dependent pro-drugs and inhibitors are being developed. Little is known, however, on the potential role of membrane-bound GSTs in drug resistance despite the fact that detoxication of cytostatic drugs and upreg...

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Binding of glutathione and an inhibitor to microsomal glutathione transferase.

Microsomal glutathione transferase is an abundant liver protein that can be activated by thiol reagents. It is not known whether the activation is associated with changed binding properties of the enzyme. Therefore the binding of GSH and an inhibitor to rat liver microsomal glutathione transferase was studied by use of equilibrium dialysis and equilibrium partition in a two-phase system. The ra...

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ژورنال

عنوان ژورنال: Acta Biochimica Polonica

سال: 2010

ISSN: 1734-154X,0001-527X

DOI: 10.18388/abp.2010_2449