Functional analysis of c-Myb protein in T-lymphocytic cell lines shows that it trans-activates the c-myc promoter.
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منابع مشابه
c-Myc trans-activates the p53 promoter through a required downstream CACGTG motif.
c-Myc and wild-type p53 have been shown to play important roles in the regulation of cellular proliferation and oncogenic transformation. We have previously shown that the p53 promoter contains a conserved consensus recognition sequence for the basic-helix-loop-helix-containing proteins, identical to the specific binding site for c-Myc/Max heterodimers. Here, we demonstrate that this element, w...
متن کاملMyb binding sites mediate negative regulation of c-myb expression in T-cell lines.
In hematopoietic cell development, the c-myb transcription factor plays an important role. c-myb mRNA is expressed at high levels in immature proliferating cells and in leukemic cells. We have investigated the regulatory role of Myb protein binding to the human c-myb promoter. Three Myb binding sites have been described at approximately 600 bp upstream of the cap site. By transient transfection...
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Steady-state mRNA levels of the protooncogene c-myb were measured by Northern blot analysis in the human colon carcinoma cell lines LoVo, the doxorubicin-resistant derivative LoVo/Dx, Colo 205, and HT 29. Overexpression of c-myb mRNA was detected in the Colo 205 cell line, probably because of gene amplification, while in human HT 29 cells c-myb was not expressed at a detectable level. Compariso...
متن کاملTransactivation of the human c-myc gene by c-Myb.
We analyse the contribution of six Myb-binding sites in the upstream c-myc sequences to transactivation by co-transfection assays. Surprisingly, deletion of the six Myb-binding sites did not influence the transactivation of c-myc by c-Myb protein. Instead, the strongest transactivation was observed with a c-myc reporter plasmid which contains only 450 bp of exon 1 including the c-myc promoter P...
متن کاملc-myc and c-myb oncoproteins during induced maturation of human myeloid and erythroid leukaemic lines.
c-myc and c-myb mRNA have been found to be tightly regulated during haemopoietic differentiation [1]. While the expression of both oncogenes is induced in cells stimulated to proliferate, c-myc in the transition from Go to G 1 [2], and c-myb in the transition from G 1 to S phase [3], expression ceases in terminally differentiating haemopoietic cell lines. This takes the form of a progressive mo...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1990
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.10.11.5747