Full-term mouse development by abolishing Zn2+-dependent metaphase II arrest without Ca2+ release
نویسندگان
چکیده
منابع مشابه
Full-term mouse development by abolishing Zn2+-dependent metaphase II arrest without Ca2+ release.
In vertebrates, a rise in intracellular free Ca(2+) (Ca(2+)(i)) levels during fertilization initiates second metaphase (mII) exit and the developmental programme. The Ca(2+) rise has long been considered to be crucial for development, but verifying this contribution would benefit from defining its role during fertilization. Here, we delineate the role of Ca(2+) release during mII exit in wild-t...
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BACKGROUND Cytoplasmic defects are thought to cause aneuploidies in oocytes and embryos and oocyte 'reconstruction' by germinal vesicle (GV) transfer may circumvent such defects. In mice 'reconstructed' oocytes undergo meiosis and fertilize normally, but early embryonic development is compromised if their ooplasm matured in vitro. This study employs sequential MII spindle and/or pronucleus (PN)...
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ژورنال
عنوان ژورنال: Development
سال: 2010
ISSN: 1477-9129,0950-1991
DOI: 10.1242/dev.049791