Frequent Detection of Respiratory Viruses by Real-Time PCR in Adenoid Samples from Asymptomatic Children

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Frequent detection of respiratory viruses by real-time PCR in adenoid samples from asymptomatic children.

In the March 2009 issue of the Journal of Clinical Microbiology, Sato et al. describe the detection of viruses in human adenoid tissue samples by using PCR assays (11). We wish to add our experience from a similar study. Thirty tissue samples were obtained from 30 individual children admitted for adenoidectomy at Bonn University Medical Centre, Department of Otorhinolaryngology. The median age ...

متن کامل

Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...

متن کامل

Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children.

Respiratory virus infections are a major health concern and represent the primary cause of testing consultation and hospitalization for young children. We developed and compared two assays that allow the detection of up to 23 different respiratory viruses that frequently infect children. The first method consisted of single TaqMan quantitative real-time PCR assays in a 96-well-plate format. The...

متن کامل

multiplex sybr green real-time pcr assay for detection of respiratory viruses

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

متن کامل

Detection of Human Influenza Viruses in Nasopharyngeal Samples by RT-PCR vs Tissue Culture

Background and Aims: Influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. A variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. Methods: Nasal and pharyngeal samples taken from patients were inoculated into Madin-Darby canine kidney (MOCK) cells and embryonated chicken eggs (ECEs). The culture media was a...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2009

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.00899-09