Fractionation of Grape Anthocyanin Classes Using Multilayer Coil Countercurrent Chromatography with Step Gradient Elution
نویسندگان
چکیده
منابع مشابه
Large-Scale Gradient Elution Chromatography
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متن کاملIsolation of flavanol-anthocyanin adducts by countercurrent chromatography.
Pigments of the flavanol-anthocyanin (F-A+) type detected earlier in wine are synthesized using a protocol adapted from the synthesis of procyanidin dimers. The F-A+ adduct thus obtained is purified by countercurrent liquid-liquid partition, currently referred to as countercurrent chromatography (CCC). The solvent system consists of tert-butyl methyl ether-n-butanol-acetonitrile-water (2:2:1:5,...
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The continuous separation of proteins was performed in a countercurrent gradient chromatography (CGC) system. A magnetically stabilized fluidized bed (MSFB) was used to establish true countercurrent contact of a solid resin with a liquid buffer. STable pH gradients were formed in the system in less than 10 min and remained stable throughout the course of the separation experiment (>2 h). The sh...
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-Gradient elution in nonlinear chromatography is a very powerful tool for the separation of components having a wide range of retentivity. It is not only used for chemical analysis, but also used in preparativeand large-scale chromatography for the separation of many macromolecules, such as proteins. In this work, a general rate model has been presented for the study of gradient elution in nonl...
متن کاملCountercurrent Chromatography and High Speed Countercurrent Chromatography: Instrumentation
Engelsma M, KokWT and Smit HC (1990) Selective determination of trace levels of phenol in river water using electrochemical concentration modulation. Journal of Chromatography 506: 201}210. Fister JC, Jacobson C and RamseyM (1999) Ultra sensitive cross correlation electrophoresis on microdevices. Analytical Chemistry 71: 4460}4464. Kaljurand M and KuK llik E (1989) Computerized Multiple Input C...
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ژورنال
عنوان ژورنال: Journal of Agricultural and Food Chemistry
سال: 2004
ISSN: 0021-8561,1520-5118
DOI: 10.1021/jf034906a