Four new yeasts in the Pichia anomala clade.
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Four new yeasts in the Pichia anomala clade.
Four new yeasts are described that were recognized as novel from nucleotide substitutions in domain D1/D2 of 26S rDNA, a region that is sufficiently divergent to allow resolution of most ascomycetous yeast species. The new species and their type strains are as follows: Pichia maclurae NRRL Y-5377T (= CBS 8671T); Pichia misumaiensis NRRL Y-17389T (= CBS 8062T); Candida mycetangii NRRL Y-6843T (=...
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Two yeast killer toxins active on spoilage yeasts belonging to the genus Dekkera/Brettanomyces are here described for the first time. The two toxins produced by Pichia anomala (DBVPG 3003) and Kluyveromyces wickerhamii (DBVPG 6077), and named Pikt and Kwkt, respectively, differ for molecular weight and biochemical properties. Interestingly, the fungicidal effect exerted by Pikt and Kwkt against...
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Synthesis of invertase (EC 3.2.1.26) in Pichia anomala is controlled by the carbon source in the culture medium. The enzyme was purified to homogeneity from P. anomala cells fully derepressed for invertase synthesis and shown to be a multimeric glycoprotein composed of identical subunits with an apparent molecular mass of 86.5 kDa. The carbohydrate moiety accounts for approx. 30% of the total m...
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Biosurfactant production by Pichia anomala PY1, a thermotorelant strain isolated from fermented food, was examined as grown in media containing various carbon and nitrogen sources. The optimal conditions for biosurfactant production included 4% soybean oil as carbon source at pH 5.5 at 30 degrees C for 7 d. Under these conditions, the surface tension of the medium decreased to 28 mN/m with oil ...
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Aflatoxins are extremely potent natural carcinogens and a major food safety concern because of potential contamination of food commodities. Threshold levels set by the U. S. Food and Drug Administration for aflatoxins in foods for domestic consumption are less than 20 parts/ billion (ppb). However, the European Union (EU) and Japan have set threshold levels at less than 4 ppb. These low toleran...
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ژورنال
عنوان ژورنال: International Journal of Systematic and Evolutionary Microbiology
سال: 2000
ISSN: 1466-5026,1466-5034
DOI: 10.1099/00207713-50-1-395