Formulations of liposomal vaccine containing AdhO36 antigen and β-glucan as immunoadjuvant

Humans are the most crucial host of Salmonella Typhi as it has limited ability to reproduce outside human body. infections caused Typhoid fever which can be prevented with vaccines. This study aims develop an effective typhoid oral vaccine using a liposomal delivery system. Several studies showed that immunization AdhO36 protein from orally provides significant protection in inhibiting mice intestine. β-Glucan is potent immunostimulant for macrophages activations. Meanwhile, method used produce Liposome formulations was thin-film hydration. The products had good characteristics based on their particle size. size results met requirement optimum intestinal absorption 399.220 + 28.095 nm Liposome+AdhO36 and 383.360 8.594 Liposome+β-glucan. Based zeta potential measurement, electrical charges three were positive, meaning liposome cationic because DDAB (Dimethyl Dioctadecyl Ammonium Bromide) component. positive charge will facilitate internalization antigen immunity cells.