Formulation of Mupirocin Adsorbed Silver Nanoparticle with Antibiofilm Agents for Enhancing Antibacterial Activity

Abstract: Introduction: Biofilm is a complex structure of microbiome having different bacterial colonies or single type cells in group, adhere to the surface produce infections. Nanoparticles (NPs) are widely used technological fields especially medicine because their antibacterial properties. Silver nanoparticles (AgNPs) possess antimicrobial and antibiofilm property against various microbial Objectives: The main objective this study was develop new formulations characterization mupirocin loaded silver with anti biofilm agents. Methods: were prepared by tannic acid (TA) trisodium citrate (TSC), Para-amino salicylic (PASA) ascorbic (ASC) as both reducing agents Staphylococcus aureus followed adsorption Mupirocin (MUP). Transmission Electron Microscope (TEM) shows that roughly spherical shape which also confirmed Atomic Force Microscopy (AFM). particle size range between 50 nm – 250 found be statistically significant (p<0.001) three AgNP formulations. In vitro drug release using dialysis membrane showed maximum 90% ± 2.343 AgNPs (TSC TA) formulation at end 7 hr entrapment 93.75%. tested for activity S. aureus. results compared one way ANOVA student t test. Conclusion: There no difference However best synergistic planktonic bacteria shown AgNP-PASA inspite its minimal when TA). Key words: aureus, nanoparticles, Mupirocin, Surface adsorption, Antibiofilm.