Formation of Acarbose Phosphate by a Cell-free Extract from the Acarbose Producer Actinoplanes sp.
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چکیده
منابع مشابه
Acarbose 7-phosphotransferase from Actinoplanes sp.: purification, properties, and possible physiological function.
A phosphotransferase which modifies the alpha-glucosidase inhibitor acarbose by phosphorylation at its 7-position was isolated from the acarbose producer Actinoplanes sp. and purified to homogeneity. The sequence of the first 20 amino acids of the enzyme was determined. The enzyme is an ATP-dependent kinase and shows high specificity for acarbose and some related compounds containing the pseudo...
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Actinoplanes sp. SE50/110 produces the α-glucosidase inhibitor acarbose, which is used to treat type 2 diabetes mellitus. To obtain a comprehensive understanding of its cellular metabolism, a genome-scale metabolic model of strain SE50/110, iYLW1028, was reconstructed on the bases of the genome annotation, biochemical databases, and extensive literature mining. Model iYLW1028 comprises 1028 gen...
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Pneumatosis cystoides intestinalis is a rare disorder with multiple submucosal or subserosal gas-filled cysts along the gastrointestinal wall. Pneumatosis intestinalis can be classified as either primary (idiopathic) or secondary. In the latter form, a causal relationship has been established with chronic obstructive pulmonary disease, amyloidosis, infectious colitis, intestinal obstruction, is...
متن کاملImproving acarbose production and eliminating the by-product component C with an efficient genetic manipulation system of Actinoplanes sp. SE50/110
The α-glucosidase inhibitor acarbose is commercially produced by Actinoplanes sp. and used as a potent drug in the treatment of type-2 diabetes. In order to improve the yield of acarbose, an efficient genetic manipulation system for Actinoplanes sp. was established. The conjugation system between E. coli carrying ØC31-derived integrative plasmids and the mycelia of Actinoplanes sp. SE50/110 was...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1996
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.49.661