Formation and regeneration of protoplasts in Sclerotium rolfsii ATCC 201126
نویسندگان
چکیده
منابع مشابه
Formation and regeneration of protoplasts in Sclerotium rolfsii ATCC 201126.
AIMS Different cultural conditions for forming and reverting protoplasts were systematically studied to establish a rapid and efficient protocol for Sclerotium rolfsii ATCC 201126. METHODS AND RESULTS Osmotic stabilizer, lytic enzymes and mycelial age were the main factors influencing protoplast yields. An optimized protocol involving 1-h hydrolysis of 45-h-old mycelium with Trichoderma harzi...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2004
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2003.02145.x